Thermo Fisher Scientific ID3 Monoclonal Antibody (OTI5C7), TrueMAB
ID3 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI5C7). Western blot, IHC, ICC, Flow Cytometry에 사용 가능. 인간 ID3 단백질에 특이적이며, 액상 형태로 제공. 연구용으로 세포 분화 및 유전자 발현 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C7 |
| Immunogen | Full length human recombinant protein of human ID3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.83 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Target Information
Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3, and ID4. They are ubiquitously expressed and form heterodimers with class A and B HLH proteins. Because ID proteins lack the basic DNA-binding region, these heterodimers cannot bind DNA, acting as negative regulators of bHLH transcription factors.
ID1 inhibits DNA binding of E12 and Myo D, thereby suppressing muscle-specific gene expression. During muscle differentiation, ID protein levels decrease, allowing E12/E47 to interact with Myo D and myogenin to activate myogenic differentiation. Expression of ID proteins is growth factor–dependent, and reduction of ID mRNA delays cell cycle reentry after growth factor stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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