Thermo Fisher Scientific ICAM-3 Monoclonal Antibody (MEM-171), Alexa Fluor 488
Alexa Fluor 488로 표지된 인간 ICAM-3(CD50) 인식 단클론 항체로, Flow Cytometry에 적합합니다. D2 도메인 외부 에피토프를 인식하며, 인간 백혈구 및 내피세포에서 발현됩니다. 액상 형태로 PBS/BSA 버퍼에 보존되며, 4°C 암소 보관이 필요합니다.
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Thermo Fisher Scientific ICAM-3 Monoclonal Antibody (MEM-171), Alexa Fluor 488
Applications
- Flow Cytometry (Flow)
Tested Dilution: 4 µL per 100 µL whole blood or 10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-171 |
| Immunogen | Human granulocytes; Target Family: CD50 (ICAM-3), UniProt ID: P32942-1, Antigen Range: 1–547 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539519 |
Additional Formats
- Unconjugated
- Biotin
- PE
- Custom conjugation available upon request
Product Specific Information
This antibody recognizes an extracellular epitope in the D2 domain of CD50 (ICAM-3), a 120–130 kDa type I membrane protein belonging to the immunoglobulin supergene family. It is expressed on leukocytes, endothelial cells, and Langerhans cells, but not on platelets or erythrocytes.
Target Information
CD50 (ICAM-3) is a transmembrane glycoprotein expressed by leukocytes and functions as a counter-receptor for the LFA-1 integrin. It mediates adhesion between T cells and antigen-presenting cells, regulates LFA-1 affinity for ICAM-1, and induces T cell activation and proliferation. ICAM-3 plays a critical role in initiating immune responses and interacts with CD209 (DC-SIGN), facilitating communication between dendritic cells and granulocytes.
Usage Notes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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