
Thermo Fisher Scientific Mouse anti-Human IgG4 Fc Secondary Antibody, HRP
HRP 결합 Mouse anti-Human IgG4 Fc 단클론 2차 항체로 Western blot, IHC, ELISA 등에 사용 가능. 고순도 정제된 형태로 높은 특이성과 감도 제공. Lyophilized 형태로 장기 보관 용이하며, 재구성 후 안정적으로 사용 가능.
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- A10654
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Thermo Fisher Scientific Mouse anti-Human IgG4 Fc Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| ELISA | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | HP6025 |
| Immunogen | Human IgG4 |
| Conjugate | HRP |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.02% thimerosal |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2534054 |
| Target | IgG4 |
| Antibody Form | Whole Antibody |
Product Specific Information
Storage and reconstitution: When stored desiccated at or below -20°C, the lyophilized powder is stable for at least one year.
Reconstitute using 200 µL PBS (pH 7.2) to yield a 1 mg/mL stock solution containing 0.02% thimerosal. After reconstitution, store at 4°C.
For prolonged storage after reconstitution, add glycerol to a final concentration of 50% (v/v), aliquot, and store at or below -20°C. When stored properly, the solution is stable for approximately three months.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in detection, sorting, or purification of their specified targets.
Secondary antibodies offer increased versatility, enabling users to employ various detection systems (e.g., HRP, AP, fluorescence).
They provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
Additional Formats
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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