Thermo Fisher Scientific Mouse anti-Human IgG4 Fc Secondary Antibody, HRP

Applications

Tested Dilution

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (IHC)

1:500-1:2,000

ELISA (ELISA)

1:500-1:2,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Secondary Antibody

Clone

HP6025

Immunogen

Human IgG4

Conjugate

HRP HRP HRP

View additional formats

• [Biotin ](/antibody/secondary/product-name/Human IgG4 Fc/conjugates/Biotin/host/Mouse/)
• Request custom conjugation

Form

Lyophilized

Purification

purified

Storage buffer

PBS

Contains

0.02% thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2534054

Target

IgG4

Antibody Form

Whole Antibody

Product Specific Information

Storage and reconstitution: when stored desiccated at or below -20°C, the lyophilized powder is stable for at least one year. Reconstitute using 200 µL PBS, pH 7.2, to yield a 1 mg/mL stock solution containing 0.02% thimerosal. After reconstitution, store product at 4°C.

For prolonged storage after reconstitution, add glycerol to a final concentration of 50% (v/v), aliquot, and store at or below -20°C. When stored properly, the solution is stable for approximately three months.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.