Takara PvuI restriction enzyme

PvuI restriction site:

CG AT | CG
GC | TA GC

Supplied buffer: K + BSA

Effect of DNA methylation: Enzyme activity is affected by CG methylase, but not dam methylase

Reaction temperature: 37°C

More Information

• Source: Escherichia coli carrying the plasmid encoding the Pvu I gene
• Concentration: 4–12 units/µl
• Substrate for unit definition: lambda DNA
• Genome DNA analysis: Flavobacterium okeanokoites genome DNA
• Ligation-recutting test: The ligation efficiency of cohesive-ended DNA fragments generated by this enzyme will be low. More efficient ligation can be achieved using blunt end ligation reaction conditions
• Each lot undergoes stringent quality testing, including overdigestion, genomic DNA analysis, ligation-recutting, and pKF3 cloning

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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