
Takara PvuI restriction enzyme
PvuI 제한효소는 CGAT|CG 절단 부위를 인식하며, CG 메틸화에 영향을 받습니다. 37°C에서 반응하며, E. coli 유래 효소입니다. 고품질 QC를 거쳐 게놈 DNA 분석 및 라이게이션 테스트에 적합합니다. 연구용으로만 사용됩니다.
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PvuI Restriction Enzyme
Restriction Site
CG AT | CG
GC | TA GC- Supplied Buffer: K + BSA
- Effect of DNA Methylation: Enzyme activity is affected by CG methylase, but not by dam methylase
- Reaction Temperature: 37°C
Specifications
| 항목 | 내용 |
|---|---|
| Source | Escherichia coli carrying the plasmid encoding the PvuI gene |
| Concentration | 4–12 units/µl |
| Substrate for Unit Definition | Lambda DNA |
| Genome DNA Analysis | Flavobacterium okeanokoites genome DNA |
| Ligation-Recutting Test | The ligation efficiency of cohesive-ended DNA fragments generated by this enzyme will be low. More efficient ligation can be achieved using blunt end ligation reaction conditions |
| Quality Testing | Each lot undergoes stringent quality testing, including overdigestion, genomic DNA analysis, ligation-recutting, and pKF3 cloning |
Additional Product Information
Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications.
Please see the Kit Components List to determine kit components.
Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliates.
Additional product and restricted use information is available at takarabio.com.
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