Thermo Fisher Scientific Precision ID Identity and Library Kit Bundle

Applied Biosystems™ Precision ID Identity and Library Kit Bundle

The Applied Biosystems™ Precision ID Identity Panel and Library Kit Bundle is designed for easy ordering and preparation of amplicon libraries using the Precision ID Identity Panel (Cat. No. A25643) and the Precision ID Library Kit (Cat. No. A26435) for sequencing on Ion Torrent™ systems.
The Precision ID Identity Panel of multiplexed SNPs provides unique identification of degraded or trace samples with discrimination similar to STR genotype match probabilities used by forensic analysts (between 1×10⁻³¹ and 6×10⁻³⁵).

주요 특징

Simple protocol

• 96 well plate-based workflow enables easy sample management
• No library amplification with qPCR quantification

Optimized digestion reagent

• Cleaner digest allows for more efficient downstream sequencing

Scalable multiplex PCR reactions

• Built on Ion AmpliSeq™ chemistry enabling scalable multiplex PCR for 124 markers using just 1 ng of DNA
• Superior coverage uniformity and specificity (performance observed down to 180 pg input)
• Proprietary primer modifications allow removal of primer sequences during library prep for efficient target assessment
• Multiple primer pools can be used to create overlapping amplicons for complete coverage of large targets

The included Precision ID Library Kit provides reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries.
It supports barcoding using the Ion Xpress™ Barcode Adapters 1–96 Kit and IonCode™ Barcode Adapters 1–384 Kit.
Barcoded libraries can be pooled and loaded onto a single Ion chip to minimize sequencing run time and cost, allowing accurate sample-to-sample comparisons.

Easy and flexible protocol

• DNA libraries are ready for downstream template preparation for clonal amplification on Ion Sphere™ particles using the automated Ion OneTouch™ 2 System
• Torrent Suite Software and HID SNP Genotyper plug-in enable rapid analysis from extracted DNA to ancestry-informative genotypes in less than two days*

* Using the Applied Biosystems™ Precision ID NGS System for Human Identification

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