Thermo Fisher Scientific PHD1 Polyclonal Antibody
Human, Mouse, Rat 반응성을 가진 Rabbit Polyclonal Antibody로 다양한 면역실험(WB, IHC, ICC, ELISA)에 사용 가능. PHD1 단백질을 표적하며 세포 성장 및 산소 항상성 연구에 적합. 고순도 Protein A 정제, 안정적 액상 형태로 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human PHD1/prolyl hydroxylase, amino acids 321–407 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2, and HIF PHD3 can hydroxylate HIF-alpha subunits. Hypoxia-inducible factor (HIF) is a transcriptional regulator critical for oxygen homeostasis. The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins.
HIF PHD1 is widely expressed, with highest levels in testis, and functions as a cellular oxygen sensor important in cell growth regulation. It can localize to the nucleus or cytoplasm and is detected in hormone-responsive tissues such as mammary, ovarian, and prostate epithelium (normal and cancerous).
HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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