Thermo Scientific™

beta-Galactosidase Assay Reagent

The Thermo Scientific Mammalian β-Galactosidase Assay Reagent is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple자세히 알아보기

The Thermo Scientific Mammalian β-Galactosidase Assay Reagent is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.

The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Features of the Thermo Scientific Mammalian β-Galactosidase Assay Kit (assay reagent and stop solution):

• Choose stand-alone assay reagent or complete kit for cell lysis and detection
• Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
• Adaptable to 96-well microplates or tube assay formats
• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatiible with prepared lysates from other species such as bacteria

Related Products
Mammalian beta-Galactosidase Assay Kit
beta-Galactosidase Assay Stop Solution

사양

어세이Reporter Enzyme

검출 방법Colorimetric

형태Liquid

용도(애플리케이션)Absorbance

제품라인Pierce™

수량25 mL

기질Beta-Gal Substrate

Substrate PropertiesChemical Substrate

Target EnzymeBeta-Galactosidase

형식96-well Plate

용도(장비)Microplate Reader

라벨 또는 염료Enzyme Labeled

제품 유형Pierce beta-Galactosidase Assay Reagent

Unit SizeEach