Thermo Fisher Scientific DNA-PK Monoclonal Antibody (42-psc (42-26, 42-27))

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 20 publications 20 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:400

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 2 publications 2 publications

ChIP assay (ChIP)

-

View 1 publication 1 publication

Neutralization (Neu)

-

View 1 publication 1 publication

in situ PLA (PLA)

-

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

42-psc (42-26, 42-27)

Immunogen

Human DNA-dependent Protein Kinase purified from HeLa cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DNA-PK,uniProtId:P78527-1,ncbiNodeId:9606,antigenRange:1-4128,antigenLength:4128,antigenImageFileName:MA5-13249_DNA-PK_P78527-1_House_mouse.svg,antigenImageFileNamePDP:MA5-13249_DNA-PK_P78527-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10987499

Product Specific Information

MA5-13249 targets DNA-PKcs in GS, IF, and IHC (P) applications and shows reactivity with Human samples.

The MA5-13249 immunogen is human DNA-dependent Protein Kinase purified from HeLa cells.

Target Information

DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.