Thermo Fisher Scientific Pan-Histone H3 Polyclonal Antibody
Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 사용 가능한 다중종 반응성 항체. 인간, 마우스, 돼지, 랫트에 반응. Rabbit polyclonal IgG로 제작되었으며, Histone H3.1 유래 펩타이드로 면역화됨. 고순도 Protein A 정제, 안정한 액상 포맷.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human Histone H3.1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.03% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it forms the nucleosome structure that organizes DNA. Histones play key roles in transcription regulation, DNA repair, replication, and chromosomal stability. The N-terminal tail of histone H3 can undergo multiple post-translational modifications, such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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