Thermo Fisher Scientific F4/80 Recombinant Rabbit Monoclonal Antibody (4J7L6)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4J7L6

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 367-645 of mouse F4/80. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:F4/80,uniProtId:Q61549-1,ncbiNodeId:10090,antigenRange:367-645,antigenLength:931,antigenImageFileName:MA5-55206_F480_Q61549-1_Rabbit.svg,antigenImageFileNamePDP:MA5-55206_F480_Q61549-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.6 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen Sequence: KRILFKCKED LILQSEQIQQ CQAVQGRDLG YASFCTLVNA TFTILDNTCE NKSAPVSLQS AATSVSLVLE QATTWFELSK EETSTLGTIL LETVESTMLA ALLIPSGNAS QMIQTEYLDI ESKVINEECK ENESINLAAR GDKMNVGCFI IKESVSTGAP GVAFVSFAHM ESVLNERFFE DGQSFRKLRM NSRVVGGTVT GEKKEDFSKP IIYTLQHIQP KQKSERPICV SWNTDVEDGR WTPSGCEIVE ASETHTVCSC NRMANLAIIM ASGELTMEF

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.