Thermo Fisher Scientific RFWD3 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Product Specifications

Product Specific Information

• Recommended positive controls: NT2D1, IMR32, MCF-7
• Predicted reactivity: Human (99%)
• Store product as a concentrated solution.
• Centrifuge briefly prior to opening the vial.

Target Information

E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair by mediating ubiquitination of the RPA complex (RPA1, RPA2, RPA3) and RAD51 at stalled replication forks, promoting homologous recombination.
Also mediates ubiquitination of p53/TP53 in the late response to DNA damage, acting as a positive regulator of p53 stability and the G1/S DNA damage checkpoint.
In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53.
[Source: UniProt]

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.