Thermo Fisher Scientific CD172a (SIRP alpha) Monoclonal Antibody (P84), APC, eBioscience
Thermo Fisher Scientific의 CD172a (SIRP alpha) 단일클론 항체(P84), APC-conjugated 제품으로 마우스 시료에 적합. Flow cytometry에 최적화되어 있으며, CD47과 상호작용하는 SIRPα 단백질 연구에 활용. 0.2 mg/mL 농도, 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 9 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | P84 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10736476 |
Available Conjugate Formats
- Alexa Fluor 488
- Biotin
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 645
- Custom conjugation available upon request
Product Specific Information
This P84 monoclonal antibody reacts with mouse CD172a (signal regulatory protein alpha, SIRPα).
CD172a is a cell surface glycoprotein with three Ig-like extracellular domains and two cytoplasmic ITIM motifs, which recruit SHP-1 and SHP-2 phosphatases.
It is expressed on monocytes, macrophages, and dendritic cells, but not on T or B lymphocytes. Neurons and CNS tissues also express CD172a.
CD47 serves as the extracellular ligand for CD172a, and this interaction influences dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis.
This monoclonal antibody has reported neutralizing activity.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse bone marrow cells, ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Antibody titration is recommended for optimal performance.
Fluorescence Properties:
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
SIRP alpha (CD172a) is a receptor-type transmembrane glycoprotein expressed on myeloid lineage cells (granulocytes, dendritic cells, macrophages, mast cells, hematopoietic stem cells).
It functions as a substrate for tyrosine kinases such as EGFR, PDGFR, src, and insulin receptor, negatively regulating receptor tyrosine kinase signaling pathways.
Binding of SIRPα to CD47 leads to ITIM phosphorylation and SHP-1/2 recruitment, modulating immune responses including inhibition of phagocytosis and T cell/DC activation.
SIRPα also regulates cellular responses to serum, growth factors, insulin, oncogenes, and adhesion, playing a role in physiological and pathological processes.
Cancer cells often overexpress CD47, activating SIRPα to evade macrophage-mediated destruction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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