Thermo Fisher Scientific ZNF304 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ZNF304. Recombinant protein control fragment (Product #RP-101284). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ZNF304,uniProtId:Q9HCX3-1,ncbiNodeId:9606,antigenRange:209-264,antigenLength:659,antigenImageFileName:PA5-62207_ZNF304_Q9HCX3-1_Rabbit.svg,antigenImageFileNamePDP:PA5-62207_ZNF304_Q9HCX3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2650056

Product Specific Information

Immunogen sequence: SLRQHQGDYD GQMLFSCGDE GKAFLDTFTL LDSQMTHAEV RPFRCLPCGN VFKEKS

Highest antigen sequence identity to the following orthologs: Mouse - 43%, Rat - 43%.

Target Information

ZNF304 acts as transcriptional regulator and plays a role in gene silencing. It probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of several tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. ZNF304 is also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) by inducing trimethylation of Lysine-27 of histone H3 (H3K27me3) in a Polycomb group (PcG) complexes-dependent manner. The protein associates at promoter regions of TSGs and mediates the recruitment of the corepressor complex containing the scaffolding protein TRIM28, methyltransferase DNMT1 and histone methyltransferase SETDB1 and/or the PcG complexes at those sites. It acts as a transcription factor involved in the metastatic cascade process by inducing cell migration and proliferation and gain resistance to anoikis of ovarian carcinoma (OC) cells via integrin-mediated signaling pathways. It associates with the ITGB1 promoter and positively regulates beta-1 integrin transcription expression. It promotes angiogenesis and tumor growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.