Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), PE-Cyanine7, eBioscience
마우스 CD25(IL-2Rα)에 특이적인 PC61.5 클론 단일클론 항체로, PE-Cyanine7 형광으로 표지되어 있습니다. Flow cytometry에 최적화되어 있으며, 0.125 µg/test로 사용 가능합니다. 488–561 nm에서 여기되고 775 nm에서 방출되며, 빛에 민감하므로 어둡게 보관해야 합니다.
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Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), PE-Cyanine7, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.125 µg/test | View 157 publications |
| Functional Assay (Functional) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, lambda |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PC61.5 |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469607 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 563, 661, 737, 805
- Brilliant Violet 421, 650, 711, 786
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue 585, 610-70S, 660-120S, 760
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage and activated mature T and B lymphocytes. It binds IL-2 with low affinity alone but forms a high-affinity receptor when associated with CD122 (IL-2Rβ) and CD132 (common γ chain).
Binding of IL-2 to both receptor classes is inhibited by the PC61.5 antibody. CD25 plays a key role in lymphocyte differentiation and activation/proliferation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes, ≤0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.
- Light Sensitivity: Tandem dye sensitive to photo-induced oxidation; protect from light.
- Fixation: Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
- Excitation / Emission / Laser: 488–561 nm / 775 nm / Blue, Green, Yellow-Green Lasers
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD25 (IL2RA) encodes the interleukin-2 receptor alpha chain, part of a heterotrimeric receptor complex with IL2RB and IL2RG.
- High-affinity receptor: IL2RA + IL2RB + IL2RG
- Low-affinity receptor: IL2RA homodimer
- Medium-affinity receptor: IL2RB homodimer
IL2 is primarily produced by mature T cells and regulates proliferation, differentiation, and apoptosis of lymphocytes. It stimulates B cell growth, enhances NK cell activity, and suppresses granulocyte macrophage colony formation. Disruption of IL2RA in mice leads to ulcerative colitis-like disease, suggesting its role in immune response regulation. Mutations are associated with interleukin-2 receptor alpha deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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