Thermo Fisher Scientific Chicken anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, AP
Thermo Fisher Scientific의 Chicken anti-Rat IgG (H+L) AP 결합 2차 항체로, Rat IgG 검출에 적합합니다. Western blot, IHC, ELISA에 사용 가능하며, 높은 특이성과 감도를 제공합니다. 교차 흡착 처리로 비특이적 결합을 최소화했습니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 1% BSA, 30 mM triethanolamine, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535513 |
| Target | IgG |
| Cross Adsorption | Against human and rabbit IgG and serum proteins |
| Antibody Form | Whole Antibody |
Available Formats
- Alexa Fluor 488
- Alexa Fluor 594
- Alexa Fluor 647
- Biotin
- HRP
- Custom conjugation available upon request
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of rat IgG targets. Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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