Thermo Fisher Scientific ZNF496 Monoclonal Antibody (4B1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

0.3 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4B1

Immunogen

ZNF496 (NP_116141, 485 a.a. approximately 585 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ZNF496,uniProtId:Q96IT1-1,ncbiNodeId:9606,antigenRange:485-585,antigenLength:587,antigenImageFileName:H00084838-M02_ZNF496_Q96IT1-1_House_mouse.svg,antigenImageFileNamePDP:H00084838-M02_ZNF496_Q96IT1-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: HLQPDRLQPV EKREQAASED ADKGPKEPLE NGKAKLSFQC CECGKAFQRH DHLARHRSHF HLKDKARPFQ CRYCVKSFTQ NYDLLRHERL HMKRRSKQAL N

Target Information

ZNF496 contains a SCAN box, a KRAB-A domain, and four consensus C2H2-type zinc fingers preceded by a unique finger derivative, referred to herein as the C2HR motif. The C2HR motif functions to mediate protein-protein interaction with the cysteine-rich (C5HCH) domain of NSD1 in a Zn(II)-dependent fashion, and when tethered to RNA polymerase II promoters, represses transcription in an NSD1-dependent manner. ZNF496 contains a novel type of zinc finger motif that functions as a docking site for NSD1 and is more than just a degenerate evolutionary remnant of a C2H2 motif.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.