Thermo Fisher Scientific RUSC1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human RUSC1. Recombinant protein control fragment (Product #RP-94359). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RUSC1,uniProtId:Q9BVN2-1,ncbiNodeId:9606,antigenRange:451-524,antigenLength:902,antigenImageFileName:PA5-55885_RUSC1_Q9BVN2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-55885_RUSC1_Q9BVN2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2646880

Product Specific Information

Immunogen sequence: LEVRSSWSFA GVPGAQRLWM AEAQSGTGQL QEQKKGLLIA VSVSVDKIIS HFGAARNLVQ KAQLGDSRLS PDVG

Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 96%.

Target Information

RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with Ikappa-B kinase- (IKK-) -gamma, the regulatory subunit of the IKK complex that is required for NF-kappa-B activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-kappa-B activation pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.