Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb1), Functional Grade, eBioscience

Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb1), Functional Grade, eBioscience™

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Request custom conjugation

Product Specific Information

Description:
The MAb1 antibody reacts with human tumor necrosis factor-alpha (TNF-alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4⁺ T cells. TNF-alpha has cytolytic activity against various tumor cells and plays a key role in immune regulation. It forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Applications Reported:
MAb1 antibody has been reported for use in ELISA, ELISPOT, intracellular staining, and neutralization.

Applications Tested:
MAb1 antibody has been tested for neutralizing TNF alpha binding and biological activity.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90%, determined by SDS-PAGE.

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).

Aggregation:
<10%, determined by HPLC.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors. TNF alpha regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It forms multimers and shows a 17 kDa band under non-reducing SDS-PAGE conditions. Knockout studies in mice suggest TNF alpha’s neuroprotective and tumor necrotic functions. It plays roles in immune responses to infections and tumor necrosis, and is implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.