Thermo Fisher Scientific ELL Monoclonal Antibody (OTI3A8)
ELL 단백질을 인식하는 마우스 단클론 항체로, WB 및 IHC(파라핀) 적용 가능. 고순도 친화 크로마토그래피 정제, 1 mg/mL 농도, 비결합형 액상 형태. 인체 및 마우스 반응성, RNA 중합효소 II/III 전사 관련 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 302–621 of ELL produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723122 |
Target Information
ELL is an elongation factor component of the super elongation complex (SEC), which enhances the catalytic rate of RNA polymerase II transcription by suppressing transient pausing at multiple DNA sites.
It is also part of the little elongation complex (LEC), regulating small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780).
ELL specifically stimulates the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription and plays an early role in stabilizing RNA polymerase II recruitment and initiation before SEC assembly.
Required for stabilization of the pre-initiation complex and early elongation.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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