Thermo Fisher Scientific PD-L2 Mouse ProcartaPlex Simplex Kit

Invitrogen™ PD-L2 Mouse ProcartaPlex™ Simplex Kit

The Mouse PD-L2 Simplex ProcartaPlex Kit measures PD-L2 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel utilizing Luminex xMAP technology for protein detection and quantitation. When combining multiple Simplex kits (i.e., when not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed per assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate multiple protein targets simultaneously using a Luminex instrument. These assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and yield results in approximately four hours.

• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single small-volume sample from plasma, serum, cell culture supernatants, and other fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures identifiable by Luminex detection systems (e.g., Luminex 200, FLEXMAP 3D, MAGPIX). Similar to a sandwich ELISA, matched antibody pairs identify the protein of interest. Each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are detected with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). This enables multiplexed analysis of multiple targets in a single well.

The key difference from a traditional ELISA is that the capture antibody in a ProcartaPlex assay is conjugated to a bead rather than adsorbed to a microplate well, allowing reagents to remain free-floating in solution. Detection is achieved using two lasers in the Luminex 200 instrument: one to distinguish bead spectral signatures and another to quantify RPE fluorescence, proportional to protein concentration. ProcartaPlex assays can profile more target proteins using less sample in the same time as a standard ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species: human, mouse, rat, nonhuman primate, porcine, and canine.

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