Thermo Fisher Scientific Phospho-Histone H2A.X (Ser139) Recombinant Rabbit Monoclonal Antibody (HisH2AXS139-1E4)
Phospho-Histone H2A.X (Ser139)을 인식하는 재조합 토끼 단일클론 항체로, Western blot 및 유세포분석에 적합합니다. 높은 특이성과 일관된 로트 간 재현성을 제공하며, HEK293 세포 발현 시스템에서 생산되었습니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Flow Cytometry (Flow) | 1–1,000 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HisH2AXS139-1E4 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho histone H2A.X |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896981 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family, forming part of the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) trigger phosphorylation at Ser139, producing gamma-H2AX. This phosphorylation can be detected via Western blot or immunofluorescence to assess DSB frequency.
H2AX phosphorylation involves phosphatidylinositol 3-kinases, including ATM, DNA-PK, and ATR. H2A.X contains 143 amino acids and is synthesized in both G1 and S phases. Two mRNA forms exist: a short replication-type (575 bases) and a longer basal-type (1600 bases). The gene maps to human chromosome 11q23.2–q23.3 and contributes to DNA structural organization and repair through MDC1 interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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