
Thermo Fisher Scientific Plasmid pBR322
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Invitrogen™
Plasmid pBR322
Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within자세히 알아보기
Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within the TetR gene. The plasmid, purified from DH10B™ E. coli, also has 14 non-selectable unique restriction endonuclease recognition sites. Unique cleavage sites for Hin_d III and _Cla I are found in the promoter of the tetracycline resistance gene. Insertion of DNA at either of these two sites usually results in loss of tetracycline resistance.
Performance and Quality Testing: Purity, DNA structure, and selected restriction endonuclease fragmentation patterns are verified by agarose gel analysis. Transformation of E. coli HB101 to confirm AmpR and TetR is confirmed by plating on selective medium.
사양
클로닝 방법Restriction Enzyme/ MCS
농도0.25 μg/μl
용도(애플리케이션)Cloning
제품 유형Plasmid
수량25 μg
배송 조건Approved for shipment on Wet or Dry Ice
벡터pBR322
Unit SizeEach
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