Thermo Fisher Scientific Rabbit anti-Human IgG Fc Secondary Antibody, Biotin
Rabbit anti-Human IgG Fc Biotin-conjugated secondary antibody for WB, IHC, ICC, Flow, ELISA, and IP applications. High specificity to human IgG heavy chains, affinity-purified, lyophilized form, stored at 4°C. For research use only.
- 카탈로그번호
- 31789
- 판매단위
- pk
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Thermo Fisher Scientific Rabbit anti-Human IgG Fc Secondary Antibody, Biotin
Applications and Tested Dilutions
| Application | Tested Dilution Range |
|---|---|
| Western Blot (WB) | 1:20,000–1:400,000 |
| Immunohistochemistry (IHC) | 1:500–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:5,000 |
| Flow Cytometry (Flow) | 1:200–1:1,000 |
| ELISA | 1:20,000–1:400,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 15 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_228407 |
| Target | IgG Fc |
| Antibody Form | Whole Antibody |
| Country of Origin | USA |
Additional Formats Available
- AP conjugate
- FITC conjugate
- HRP conjugate
- Custom conjugation available upon request
Product Specific Information
Concentration may vary slightly between lots; refer to lot-specific datasheet for exact concentration.
Product #31789 has been validated in Western blot, IF, ICC, IHC, IP, and FACS applications.
This antibody reacts with the heavy chains of human IgG but not with light chains common to most human immunoglobulins. It does not react against human IgM or IgA, or non-immunoglobulin serum proteins. Cross-reactivity with immunoglobulins from other species may occur.
Store at 4°C until opened. To extend shelf-life, add an equal volume of glycerol (final ~50%) and store at -20°C.
Reconstitute with 1.5 mL of distilled water before use.
Target Information
Anti-Human secondary antibodies are affinity-purified reagents with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies offer versatility and increased sensitivity through signal amplification, as multiple secondary antibodies can bind to one primary antibody.
Typically, they are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to enhance specificity.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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