Thermo Fisher Scientific Aquaporin 2 Polyclonal Antibody
Aquaporin 2 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC(P) 분석에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 냉장 또는 -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1.0 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | 19 amino acid peptide near the carboxy terminus of human AQP2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2554608 |
Product Specific Information
- A recommended positive control is A431 cell lysate.
- This antibody is not expected to cross-react with other members of the aquaporin protein family.
Target Information
Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct.
Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans.
A vasopressin-induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from intracellular vesicles to the apical membrane of principal cells.
Serine-261 has been identified as a novel phosphorylation site, with levels of phosphorylated S261 decreasing upon vasopressin treatment, suggesting its involvement in vasopressin-dependent AQP2 trafficking.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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