Invitrogen™

ATP Determination Kit

Detect and quantify ATP in biochemical and cell viability assays with the ATP Determination Kit. This luminescence-based ATP assay utilizes the luciferase-luciferin reaction, offering exceptional sensitivity to detect as little as 0.1 picomole of ATP using a luminometer or microplate reader.

The ATP Determination Kit is a highly sensitive luminescence assay designed for the quantitative detection of ATP levels in biochemical and cell viability assays. This ATP assay kit utilizes the reaction between recombinant firefly luciferase and its substrate D-luciferin and is based on luciferase`s absolute requirement for ATP in producing light (emission maximum approximately 560 nm at pH 7.8). With its exceptional sensitivity, capable of detecting as little as 0.1 picomole of ATP, this assay is well-suited for kinetic studies and can be used with luminometers or microplate readers equipped with a luminescence mode, such as the Thermo Scientific Varioskan ALF and LUX Multimode Microplate Readers.

This luciferase ATP assay includes sufficient reagents for ten 96-well plates and offers a unique advantage over other commercially available ATP detection kits by providing the luciferase and luciferin components separately, allowing researchers to optimize the reaction conditions for their specific instruments and samples.

사양

검출 방법Bioluminescence, Chemiluminescence

용도 (장비)Luminometer (Microplate), Luminometer (Tube-based), Microplate Reader

제품 유형ATP Determination Kit

형식Tube, 96-well plate

수량1 Kit

배송 조건Wet Ice

기질D-Luciferin

충분200–1000 assays

라벨 유형Luciferin

Unit SizeEach