Takara Stellar Electrocompetent Cells for cloning

Our Stellar Electrocompetent Cells provide high transformation efficiency, making them suitable for many molecular biology applications, such as generation of cDNA libraries or constructing gene banks. This E. coli strain can be used in place of DH10B cells and has several useful features. The r_ecA_1 and endA_1 markers in the strain minimize recombination and degradation of plasmid DNA. These electrocompetent cells, based on the HST08 _E. coli strain, provide alpha-complementation of the beta-galactosidase gene for blue/white screening of recombinant colonies (positive clones).

Stellar Electrocompetent Cells lack the gene cluster which digests foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA) and are therefore useful for cloning of methylated DNA, in addition to the construction of standard and longer-length genomic libraries.

More Information

Transformation efficiency

>1 × 109 cfu/µg

Genotype

F-, endA1, supE44, thi-1, recA1, relA1, gyrA96, phoA, Φ80d lacZΔ M15, Δ (lacZYA - argF) U169, Δ (mrr - hsdRMS - mcrBC), ΔmcrA, λ-

Applications

• Cloning of large fragments
• cDNA library construction
• Genomic library construction
• Long-length genomic library construction

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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