Thermo Fisher Scientific YAP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

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Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

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Immunocytochemistry (ICC/IF)

1:100-1:1,000

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Immunoprecipitation (IP)

1:100-1:500

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ChIP assay (ChIP)

2.5 µg/10^6 cells

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human YAP1. The exact sequence is proprietary. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:YAP1,uniProtId:P46937-1,ncbiNodeId:9606,antigenRange:1,antigenLength:504,antigenImageFileName:PA5-78321_YAP1_P46937-1_Rabbit.svg,antigenImageFileNamePDP:PA5-78321_YAP1_P46937-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2736810

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2

Predicted Reactivity: Mouse (96%), Rat (96%), Zebrafish (87%), Xenopus laevis (81%), Chicken (84%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.