Thermo Fisher Scientific GLE1 Monoclonal Antibody (1D8)
Human GLE1 단백질을 인식하는 Mouse IgG1 단일클론 항체. Western blot, ICC/IF, ELISA에 사용 가능. Affinity chromatography로 정제된 액상 형태이며, PBS(pH 7.4) 버퍼에 보존제 없이 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.1 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D8 |
| Immunogen | GLE1 (NP_001003722.1, 140 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
RMKGTEGLRL WQEEQERKVQ ALSEMASEQL KRFDEWKELK QHKEFQDLRE VMEKSSREAL GHQEKLKAEH RHRAKILNLK LREAEQQRVK QAEQERLRKEE
Target Information
The proper expression of gene products in eukaryotic cells relies on efficient transport of mRNA molecules out of the nucleus. Gle1 is an essential mRNA export factor in both human and yeast cells. It associates with the nuclear pore complex (NPC) through hCG1 and NUP155 in mammalian cells and, in conjunction with inositol hexakisphosphate (IP6), stimulates Dbp5, a member of the DEAD-box helicase family, triggering mRNP remodeling and facilitating RNA export from the nucleus.
Recent evidence suggests that mutations in Gle1 causing defects in mRNA export can result in human disease. At least three isoforms of Gle1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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