Thermo Fisher Scientific ARAF Monoclonal Antibody (OTI2F8), TrueMAB
Human ARAF 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB 및 IHC(P) 실험에 적합. 1 mg/mL 농도의 액상 형태이며, PBS/BSA/glycerol buffer에 보관. 신호전달 연구용으로 사용되며, -20°C에서 보관 권장.
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Applications
- Western Blot (WB): 1:500
- Immunohistochemistry (Paraffin) (IHC (P)): 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 145–365 of human ARAF produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is broadly expressed in nearly all cell lines tested. It is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus.
Unregulated kinase activity of v-Raf is associated with transformation and mitogenesis, while Raf-1 activity is normally suppressed by its N-terminal domain.
A-Raf, a second member of the Raf gene family, shows substantial homology to Raf-1 in the kinase domain but less elsewhere. Expression of A-Raf is highest in urogenital tissues and kidney, and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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