Thermo Scientific™

Maxima Probe/ROX qPCR Master Mix (2X)

**********************Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we`ve자세히 알아보기

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we`ve taken the best of Maxima Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (see Reference 1). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

Highlights

• Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity—detects low copy number targets
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2X master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes
Maxima Probe/ROX qPCR Master Mix (2X) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima Probe/ROX qPCR Master Mix (2X)
Maxima Probe qPCR Master Mix (2X), with separate ROX vial

사양

검출 방법Primer-probe

형식Tube

GC-Rich PCR PerformanceHigh

PCR 방법qPCR

중합효소Taq DNA Polymerase

반응 속도Standard

용도(장비)7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System

제품라인Maxima

제품 유형qPCR Master Mix

수량200 reactions

충분200 Reactions of 25 μL

용도(애플리케이션)Gene Expression

농도2X

반응 수200 reactions

샘플 종류DNA, dsDNA

부피2 x 1.25 mL

Unit SizeEach