Thermo Fisher Scientific Precision ID GlobalFiler NGS STR Panel v2

Applied Biosystems™ Precision ID GlobalFiler™ NGS STR Panel v2

The Applied Biosystems Precision ID GlobalFiler NGS STR Panel v2 enables an innovative next-generation sequencing (NGS) approach to the analysis of DNA mixtures for forensic casework applications. It uses massively parallel sequencing built on Ion AmpliSeq™ technology for superior efficiency.

This panel includes 35 markers:

• 21 CODIS STR markers
• 9 additional multi-allelic STR markers
• 4 sex determination markers

These markers aid in mixture interpretation of complex casework samples.

Using a minimal DNA input of 125 pg, this targeted forensic marker panel is optimized for use with:

• Precision ID DL8 Kit (Cat. No. A33212)
• Ion S5 Precision ID Chef & Sequencing Kit (Cat. No. A33208)

The automated Precision ID workflow enables streamlined sample extraction-to-genotypes using:

• Ion Chef™ System
• Ion GeneStudio™ series sequencer
• Converge™ Software NGS Analysis Module

Key Features

Simplicity

• Requires as little as 125 pg of input DNA
• Supports both manual and automated Ion Chef library preparation
• Template preparation with the Ion Chef System
• Profile interpretation with the Converge NGS Analysis Module

Scalability

• Compatible with Ion 520 or Ion 530 chips
• Multiplex up to 32 samples for efficient processing

Advanced Data Analysis

The Converge Software NGS module provides complete NGS data analysis of targets amplified with the Precision ID GlobalFiler NGS STR Panel v2.
STR markers are displayed as both allele number and base sequence, improving mixture resolution.
The software also analyzes SNPs in flanking regions of STR markers.

This unique NGS STR panel combines:

• Compatibility with CODIS core loci databasing standards
• Superior discrimination power
• Optimized performance for forensic DNA laboratories

Compatibility

• Compatible with Ion S5/S5XL and Ion GeneStudio series systems
• Not compatible with Ion OneTouch2 System or Ion PGM System

Note

The GlobalFiler NGS STR Panel v2 provides sufficient material for:

• 96 reactions using the manual Precision ID Library Kit (Cat. No. A26435)
• 32 reactions using the automated Precision ID DL8 Library Kit (Cat. No. A33212)

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