Thermo Fisher Scientific IL-15/IL-15R Complex Monoclonal Antibody (GRW15PLZ), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent

-

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

T-Cell Activation (TCA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

GRW15PLZ

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2866318

Product Specific Information

Description: The monoclonal antibody GRW15PLZ recognizes the mouse IL-15/IL-15 receptor complex. Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that is has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.

Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted when in complex with its unique receptor, IL-15R alpha. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.

The monoclonal antibody GRW15PLZ reacts with and inhibits the bioactivity of IL-15 and the IL-15/IL-15R complex.

Applications Reported: The monoclonal antibody GRW15PLZ reacts with and inhibits the bioactivity of IL-15 and the IL-15/IL-15R complex.

Applications Tested: The ND50 of GRW15PLZ, as determined by the inhibition of CTLL-2 cell proliferation, is 15-60 ng/mL in the presence of 6 ng/mL recombinant mouse IL-15/IL-15R complex, or 0.4-0.8 µg/mL in the presence of 120 ng/mL recombinant mouse IL-15. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.