Thermo Fisher Scientific Ki67 Monoclonal Antibody (OTI13B11), TrueMAB
인간 Ki-67 단백질을 인식하는 Mouse monoclonal antibody로, 세포 증식 마커 연구에 적합. IHC(P)에서 1:100–1:500 희석으로 사용 가능. 고순도 Affinity chromatography 정제, PBS/BSA/glycerol buffer에 보관. 연구용으로만 사용.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI13B11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1160–1493 of human MKI67 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, especially in late G1, S, G2, and M phases. It associates with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus via its forkhead-associated (FHA) domain. The protein shows cell cycle–dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase. Ki-67 is widely used as a proliferation marker since it is absent in G0 cells and increases from G1 through mitosis. Ki-67 antibodies help determine the growth fraction in neoplasms, with prognostic value comparable to thymidine-labeling index. Low Ki-67 index correlates strongly with low-grade tumors, and it is routinely used as a neuronal marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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