Thermo Fisher Scientific NEDD8 Polyclonal Antibody
NEDD8 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB 및 ELISA에 사용 가능. Mouse와 Rat 시료 반응. 고순도 친화 크로마토그래피 정제, 액상 형태로 제공되며 -20°C 보관. 세포주기 및 단백질 분해 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–81 of human NEDD8 (NP_0061471) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.51 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804727 |
Product Specific Information
- Immunogen sequence:
MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM NDEKTAADYK ILGGSVLHLV LALRGGGGLR Q - Positive Samples: Mouse brain, Mouse kidney, Rat kidney, Rat brain
- Cellular Location: Nucleus
Target Information
NEDD8 is a ubiquitin-like protein involved in cell cycle control and embryogenesis. Its covalent attachment to substrates requires activation by the E1 complex (UBE1C-APPBP1) and linkage to the E2 enzyme (UBE2M). NEDD8 conjugation to cullins activates their associated E3 ubiquitin ligase activity, promoting polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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