Thermo Fisher Scientific NFS1 Polyclonal Antibody
Thermo Fisher Scientific의 NFS1 폴리클로날 항체는 인간, 마우스, 랫트 시료에 반응하며 WB, IHC, ICC/IF, ELISA에 적합합니다. Affinity chromatography로 정제된 고품질 항체로, PBS 및 글리세롤 용액 형태로 제공되며 -20°C에서 보관 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 290–457 of human NFS1 (NP_0669233) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.07 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901694 |
Product Specific Information
- Positive Samples: SH-SY5Y, HepG2, BxPC-3, Mouse brain, Mouse liver, Mouse heart, Rat brain, Rat liver, Rat heart
- Immunogen Sequence:
GMRSGTVPTP LVVGLGAACE VAQQEMEYDH KRISKLSERL IQNIMKSLPD VVMNGDPKHH YPGCINLSFA YVEGESLLMA LKDVALSSGS ACTSASLEPS YVLRAIGTDE DLAHSSIRFG IGRFTTEEEV DYTVEKCIQH VKRLREMSPL WEMVQDGIDL KSIKWTQH
Target Information
NFS1 (Nitrogen Fixation 1), also known as NIFS or IscS (cysteine desulfurase), belongs to the class V pyridoxal-phosphate-dependent aminotransferase family.
It localizes to the cytoplasm or mitochondrion depending on cytosolic pH.
Highest expression levels are found in heart and skeletal muscle, with lower expression in liver, brain, and pancreas.
NFS1 catalyzes the removal of sulfur from cysteine to form alanine, providing inorganic sulfur for iron-sulfur (Fe-S) clusters.
Fe-S clusters are essential cofactors in various biological processes, including:
- Electron transfer in oxidative phosphorylation
- Enzymatic catalysis in aconitase and dehydratases
- Structural maintenance of DNA repair enzyme endonuclease III
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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