Thermo Fisher Scientific NFS1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 290-457 of human NFS1 (NP_0669233) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NFS1,uniProtId:Q9Y697-1,ncbiNodeId:9606,antigenRange:290-457,antigenLength:457,antigenImageFileName:PA5-117064_NFS1_Q9Y697-1_Rabbit.svg,antigenImageFileNamePDP:PA5-117064_NFS1_Q9Y697-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.07 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901694

Product Specific Information

Positive Samples: SH-SY5Y, HepG2, BxPC-3, Mouse brain, Mouse liver, Mouse heart, Rat brain, Rat liver, Rat heart

Immunogen sequence: GMRSGTVPTP LVVGLGAACE VAQQEMEYDH KRISKLSERL IQNIMKSLPD VVMNGDPKHH YPGCINLSFA YVEGESLLMA LKDVALSSGS ACTSASLEPS YVLRAIGTDE DLAHSSIRFG IGRFTTEEEV DYTVEKCIQH VKRLREMSPL WEMVQDGIDL KSIKWTQH

Target Information

NFS1 (nitrogen fixation 1), also known as NIFS or IscS (cysteine desulfurase), is a member of the class V pyridoxal-phosphate-dependent aminotransferase family. It localizes to the cytoplasm or mitochondrion depending on which form is generated based on cytosolic pH. Highest expression levels of NFS1 are found in heart and skeletal muscle. Lower levels of expression are also found in liver, brain and pancreas. NFS1 is responsible for catalyzing the removal of sulfur from cysteine to form alanine, thereby supplying the inorganic sulfur for iron-sulfur (Fe-S) clusters. Fe-S clusters function as essential cofactors in a wide variety of events, including facilitation of electron transfer processes in oxidative phosphorylation, catalysis of enzymatic reactions in aconitase and dehydratases and maintenance of structural integrity in the DNA repair enzyme endonuclease III.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.