Thermo Fisher Scientific Phospho-Histone H2A.X (Thr120) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:2,000

Product Specifications

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome core particle. DNA double-strand breaks (DSBs) trigger phosphorylation of serine 139 at the C-terminus of H2AX, resulting in gamma-H2AX formation. This phosphorylation event can be detected by Western blotting or immunofluorescence to assess DSB frequency.
Phosphatidylinositol 3-kinases are implicated in H2AX phosphorylation, though the roles of ATM, DNA-PK, and ATR remain under investigation. Structurally, H2A.X contains 143 amino acids and is synthesized in both G1 and S phases. Its mRNA exists in two forms: a ~1600-base polyA-containing form and a ~575-base non-polyA form. The short form behaves as a replication-type histone mRNA, while the longer acts as a basal-type.
Histone H2A.X maps to the 11q23.2–q23.3 region of the human chromosome and contributes to chromatin structure and DNA organization. The H2A.X variant regulates MDC1-mediated DNA damage response signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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