Thermo Fisher Scientific WEE2 Polyclonal Antibody, FITC
FITC로 표지된 WEE2 폴리클로날 항체로, Western blot, ELISA, IP에 적합합니다. 마우스 및 랫트 반응성이 있으며, 면역원은 WEE2 단백질의 60-110 아미노산 영역에서 유래했습니다. 액상 형태로 -20°C 암소 보관이 필요합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 60–110 on mouse Wee1-like protein kinase isoform 2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1/CDC2 and acts as a key regulator of meiosis during both prophase I and metaphase II (PubMed:29606300).
Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage by phosphorylating CDK1 at Tyr-15, leading to inhibition of CDK1 activity and preventing meiotic reentry.
Also required for metaphase II exit during egg activation by phosphorylating CDK1 at Tyr-15, ensuring exit from meiosis and promoting pronuclear formation.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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