Thermo Fisher Scientific CD95 (APO-1/Fas) Monoclonal Antibody (DX2), FITC, eBioscience
인간 및 개 CD95(Fas/APO-1)를 인식하는 DX2 단클론 항체로, FITC 형광 표지되어 Flow Cytometry 분석에 최적화됨. 세포 사멸 경로 연구에 적합하며, 고순도 친화 크로마토그래피 정제 및 안정적 PBS/BSA 완충액 보존.
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Thermo Fisher Scientific CD95 (APO-1/Fas) Monoclonal Antibody (DX2), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 11 publications |
| ChIP assay (ChIP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DX2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11044915 |
Available Formats
- Unconjugated
- APC
- eFluor 450
- NovaFluor Blue 660-120S
- NovaFluor Red 700
- NovaFluor Yellow 610
- NovaFluor Yellow 700
- NovaFluor Yellow 755
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 600
- Custom conjugation available
Product Specific Information
The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40–50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells, including monocytes, neutrophils, lymphocytes, and fibroblasts. Interaction of CD95 with FasL induces apoptosis and plays a role in peripheral tolerance. DX2 does not block EOS9.1 binding, another antibody specific for human CD95.
DX2 monoclonal antibody is also reported to recognize canine CD95.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometry on normal human peripheral blood cells. Use at 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein functioning as a cell death receptor in the TNFR superfamily. It also exists in soluble form (~26 kDa). FAS stimulation aggregates FADD domains forming the DISC complex, activating caspases and triggering apoptosis.
FAS can activate NF-κB, MAPK3/ERK1, MAPK8/JNK, and participates in proliferative signaling in fibroblasts and T cells. Multiple splice variants exist, with soluble isoforms potentially regulating apoptosis negatively.
The Fas/FasL system is implicated in diseases such as AIDS, hepatitis, and cancer, and plays a crucial role in antiviral immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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