Thermo Fisher Scientific beta Actin Monoclonal Antibody (AC-15)
베타 액틴 단백질 발현 분석용 단클론 항체로, Western blot, 면역형광, 면역조직화학 등에 사용 가능. 다양한 종에서 반응하며 포르말린 고정 및 파라핀 포매에도 안정적. 세포 구조 및 단백질 발현 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 109 publications |
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 3 publications |
| Flow Cytometry (Flow) | - | View 2 publications |
| Miscellaneous PubMed (Misc) | - | View 15 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Fruit fly, Cat, Guinea pig, Human, Sheep, Pig, Rabbit |
| Published Species | Bacteria, Bovine, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Sheep, Virus, Zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AC-15 |
| Immunogen | A slightly modified synthetic beta-cytoplasmic actin N-terminal peptide (Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys) conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 15 mM sodium azide |
| Storage Conditions | 2–8°C for up to 1 month; for long-term storage, store at -20°C |
| Shipping Conditions | Dry ice |
| RRID | AB_2536382 |
Product Specific Information
The anti-beta-actin monoclonal antibody is used to analyze beta-actin protein expression in experimental cells by immunofluorescence and western blot. It has been used with beta-actin siRNA control to study RNA interference (RNAi) effects at the protein level.
This antibody reacts with beta-actin expressing cells in human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, leech, and fruit fly tissues, but not in amoeba.
Use Anti-beta-actin for detection of beta-actin by ELISA, immunohistology, western blotting, and immunofluorescence. The antibody specifically recognizes beta-actin in a wide variety of tissues and species using immunoblotting, immunofluorescent staining of cultured cell lines, and immunohistochemistry. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Ethanol, B5, methacarn, or Bouin’s solutions may also be used as fixatives.
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein involved in cell motility, structure, and integrity. Beta and gamma actins coexist in most cell types as cytoskeletal components. Beta-actin polymerizes from globular actin (G-actin) into filamentous actin (F-actin), forming a two-stranded helix.
Actins are highly conserved proteins essential for cellular movement and structure. Because beta actin is ubiquitously expressed in eukaryotic cells, it is commonly used as a loading control in protein detection assays such as Western blotting. Antibodies to beta-actin are valuable tools for studying intracellular distribution and cytoskeletal dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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