Thermo Fisher Scientific beta Actin Monoclonal Antibody (AC-15)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

The anti-beta-actin monoclonal antibody is used to analyze beta-actin protein expression in experimental cells by immunofluorescence and western blot. It has been used with beta-actin siRNA control to study RNA interference (RNAi) effects at the protein level.

This antibody reacts with beta-actin expressing cells in human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, leech, and fruit fly tissues, but not in amoeba.

Use Anti-beta-actin for detection of beta-actin by ELISA, immunohistology, western blotting, and immunofluorescence. The antibody specifically recognizes beta-actin in a wide variety of tissues and species using immunoblotting, immunofluorescent staining of cultured cell lines, and immunohistochemistry. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Ethanol, B5, methacarn, or Bouin’s solutions may also be used as fixatives.

Target Information

Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein involved in cell motility, structure, and integrity. Beta and gamma actins coexist in most cell types as cytoskeletal components. Beta-actin polymerizes from globular actin (G-actin) into filamentous actin (F-actin), forming a two-stranded helix.

Actins are highly conserved proteins essential for cellular movement and structure. Because beta actin is ubiquitously expressed in eukaryotic cells, it is commonly used as a loading control in protein detection assays such as Western blotting. Antibodies to beta-actin are valuable tools for studying intracellular distribution and cytoskeletal dynamics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.