Thermo Scientific™

RevertAid™ H Minus First Strand cDNA Synthesis Kit, with DNase I

The Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit (with DNase I) is a complete system for efficient자세히 알아보기

The Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit (with DNase I) is a complete system for efficient synthesis of first-strand cDNA from RNA templates. The kit includes recombinant endonuclease (DNase I) to remove contaminating genomic DNA from RNA preps.

For reverse transcription the kit uses RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Key features
• Integrated genomic DNA removal step
• High yields of full-length first-strand cDNA up to 13 kb
• Increased reaction temperatures in the range of 42 to 55°C
• Supplied with the recombinant RiboLock RNase Inhibitor
• Complete kit—oligo(dT)18 and random hexamer primers included with the kit

Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

사양

형식Kit

반응 속도60 min.

기술Reverse Transcription

최적 반응 온도42°C to 45°C

수량20 reactions

역전사 효소RevertAid

리보뉴클레아제 H 활성Reduced

용도(애플리케이션)Real Time PCR (qPCR), RT-PCR

최종 제품 유형First-Strand cDNA

반응 수20 Reactions

반응 형식Separate components

시약 유형Reverse Transcription

크기(최종 제품)Up to 13 kb

시작 물질RNA

Unit SizeEach