Thermo Fisher Scientific C/EBP alpha Polyclonal Antibody
C/EBP alpha 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IP, ChIP 등 다양한 응용에 적합. 인간 및 생쥐에 반응하며, 항원 친화 크로마토그래피로 정제됨. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunoprecipitation (IP) | 1:100–1:500 | View 1 publication |
| ChIP assay (ChIP) | Assay-Dependent | View 2 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human C/EBP alpha. The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.59 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735541 |
Product Specific Information
- Positive Control: THP-1, HL-60, C2C12, HepG2, NB4, U937, THP-1, MV4-11
- Predicted Reactivity: Rat (100%), Sheep (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The C/EBP (CCAAT/enhancer binding protein) family of transcription factors—alpha, beta, delta, and epsilon—are widely expressed proteins found in various tissues. These domains contain an evolutionarily conserved five-amino acid motif (RDM) with the consensus sequence (I/V/L)KXEP.
C/EBP proteins play key roles in metabolic regulation in liver and fat cells and act in cascades aiding adipose cell development.
Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display distinct functions in gene regulation.
C/EBPs have also been implicated in nerve growth factor mediation, synaptic plasticity, and inflammatory response in Alzheimer’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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