Thermo Fisher Scientific C/EBP alpha Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:100-1:500

View 1 publication 1 publication

ChIP assay (ChIP)

Assay-Dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Bovine

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human C/EBP alpha. The exact sequence is proprietary. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:C/EBP alpha,uniProtId:P49715-1,ncbiNodeId:9606,antigenRange:1,antigenLength:358,antigenImageFileName:PA5-77911_CEBP_alpha_P49715-1_Rabbit.svg,antigenImageFileNamePDP:PA5-77911_CEBP_alpha_P49715-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.59 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2735541

Product Specific Information

Positive Control: THP-1, HL-60, C2C12, HepG2, NB4, U937, THP-1, MV4-11

Predicted Reactivity: Rat (100%), Sheep (100%), Bovine (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues. These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP. One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells. Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.