Thermo Fisher Scientific AMACR/p504S Monoclonal Antibody (AMACR, 1864)
AMACR/p504S 단클론 항체로 인간 시료에 반응하며, Western blot 및 IHC(Paraffin) 검증 완료. 단백질 A/G로 정제된 액상 항체로 4°C 보관. 전립선암 조직에서 높은 발현을 검출 가능.
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Thermo Fisher Scientific AMACR/p504S Monoclonal Antibody (AMACR, 1864)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AMACR, 1864 |
| Immunogen | Recombinant human AMACR protein fragment (around aa 297–394) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
P504S/AMACR (α-Methylacyl-CoA Racemase) is an essential enzyme in the β-oxidation of branched-chain fatty acids.
High expression of P504S protein is found in prostatic adenocarcinoma but not in benign prostate tissue by immunohistochemical staining in paraffin-embedded tissue.
Expression of P504S is also detected in two premalignant lesions of the prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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