Thermo Fisher Scientific Phospho-BCKDHA (Ser292) Recombinant Rabbit Monoclonal Antibody (BLR060F)
Phospho-BCKDHA (Ser292) 단백질을 인식하는 재조합 토끼 단클론 항체. Western blot에 최적화되어 있으며, 인간 및 마우스 시료에 반응. HEK293 세포에서 발현된 IgG 형식의 항체로, 4°C에서 보관. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested dilution: 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR060F |
| Immunogen | Peptide surrounding phosphorylated serine 292 of mitochondrial human Branched Chain Keto Acid Dehydrogenase E1, Alpha polypeptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 40 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 7.8–8.6, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine.
This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998).
The BCKD complex consists of three catalytic components:
- A heterotetrameric (α2-β2) branched-chain α-keto acid decarboxylase (E1)
- A homo-24-meric dihydrolipoyl transacylase (E2)
- A homodimeric dihydrolipoamide dehydrogenase (E3)
E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains two regulatory enzymes, a kinase and a phosphorylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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