Thermo Fisher Scientific Bovine TNF-alpha Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 4 publications 4 publications

Product Specifications

Species

Bovine

Published species

Not Applicable

Expression System

P. pastoris

Amino acid sequence

Bovine TNF-alpha, amino acids 77-233 (Accession # Q06599)

Molecular weight

17.4 kDa

Class

Recombinant

Type

Protein

Activity

The biological activity of recombinant bovine TNF alpha was measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells. The ED50 for this effect is typically 5-8 ng/mL.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.

Activity: The biological activity of recombinant bovine TNF alpha was measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells. The ED50 for this effect is typically 5-8 ng/mL.

Expression: The mature, soluble form of bovine TNFa was expressed in Pichia pastoris, KM71H and therefore does not have endotoxin.

Reconstitute with 0.5 mL of distilled water.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.