Thermo Fisher Scientific CD133 Monoclonal Antibody (5E3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5E3

Immunogen

Recombinant full length human CD133 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD133 (Prominin-1),uniProtId:O43490-1,ncbiNodeId:9606,antigenRange:1-865,antigenLength:865,antigenImageFileName:MA5-18323_CD133_Prominin-1_O43490-1_House_mouse.svg,antigenImageFileNamePDP:MA5-18323_CD133_Prominin-1_O43490-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2539686

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 133 kDa and does not cross-react with related proteins.

Purity is >95% by SDS-PAGE.

Target Information

The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.