Thermo Scientific™

FastDigest BfoI

5R G C G C ↓Y 3 3Y ↑C G C G R 5 Thermo Scientific FastDigest BfoI자세히 알아보기

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5`			R	G	C	G	C ↓	Y		3`
3`			Y ↑	C	G	C	G	R		5`

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Thermo Scientific FastDigest BfoI restriction enzyme recognizes RGCGC^Y site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: HaeII, AccB2I, Bme142I, BsmHI, Bsp143II, Bst16I, Bst1473II, BstH2I, Btu34II, HinHI, LpnI, NgoAI, NgoBI, NgoCI, NgoGI, NgoJI, NgoMI, NgoWI.

Thermo Scientific FastDigest BfoI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

사양

메틸화 민감도CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive

효소BfoI

함께 사용가능한 버퍼10X FastDigest Buffer/FastDigest Green Buffer

열 불활성화에 대한 민감성Yes

최적 반응 온도37°C

Type IIS RENo

수량200 μL (200 Reactions)

제품 유형Restriction Enzyme

연구 카테고리Traditional Cloning

제품라인FastDigest™

오버행3` protruding ends

Inactivation Temperature65°C

Unit SizeEach