Thermo Fisher Scientific Collagen IV Monoclonal Antibody (1042), Alexa Fluor 647, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

1042

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

View additional formats

• Unconjugated
• Alexa Fluor 488
• eFluor 660
• Request custom conjugation

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854267

Product Specific Information

Description: This monoclonal antibody 1042 recognizes human collagen Type IV. Collagens are the main components of the extracellular matrix and provide the tensile strength to tissues. There are four main types of collagen (I, II, III and IV) which contribute to the connective tissue. Expression of type IV is restricted to the basal lamina. It contains two alpha-1 and one alpha-2-like chains. Collagen Type IV is associated with disorders such as Alport syndrome and Goodpasture`s syndrome.

Applications Reported: This 1042 antibody has been reported for use in immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (IHC-P) tissue sections.

Applications Tested: This 1042 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) tissue with low pH antigen retrieval. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.