Thermo Fisher Scientific Phospho-BTK (Tyr223) Polyclonal Antibody
BTK 단백질의 Tyr223 인산화 부위를 인식하는 폴리클로날 항체로, 인간 및 마우스 시료에 반응합니다. IHC, ICC, Flow Cytometry, ELISA 등 다양한 응용에 적합하며, 고순도 Protein A 정제 및 안정적인 액상 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human Btk around the phosphorylation site of Tyr223 [AL (p-Y)DY] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Bruton tyrosine kinase (BTK) is a cytoplasmic tyrosine kinase belonging to the SRC-related TEC subfamily of tyrosine kinases. Mutations in the BTK gene are linked to severe developmental blocks in human B-cell ontogeny and immunodeficiency disorders. BTK interacts with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9, which are critical in innate and adaptive immunity. It also associates with key proteins involved in TLR4 signal transduction, including MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting its role in lipopolysaccharide signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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