Thermo Fisher Scientific TRPM7 Polyclonal Antibody
TRPM7 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot, IHC, ICC에 적합합니다. 인간, 생쥐, 랫트 반응성이 있으며 항원 친화 크로마토그래피로 정제되었습니다. 동결건조 형태로 제공되며 재구성 후 단기 보관은 4°C, 장기 보관은 -20°C에서 권장됩니다.
- 카탈로그번호
- ACC-047-xxxxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TRPM7 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CKRRKKDKTSDGPKLFLTEE, corresponding to amino acid residues 1146–1165 of human TRPM7, Intracellular, C-terminus |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution: 25 µL, 50 µL, or 0.2 mL of double distilled water (DDW), depending on sample size.
- Ships as a lyophilized powder at room temperature.
- Upon arrival, store at -20°C.
- After reconstitution, store at 4°C for up to 1 week.
- For long-term storage, aliquot and store at -20°C.
- Avoid multiple freeze/thaw cycles.
- Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
TRPCs are mammalian homologs of the Drosophila transient receptor potential (trp) protein and function as ion channels mediating calcium entry into cells. TRPM7 (also known as TRP-PLIK) is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it can phosphorylate itself and other substrates. The kinase activity is essential for channel function and depends on intracellular ATP, as demonstrated by kinase mutants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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