Thermo Fisher Scientific BRAF Monoclonal Antibody (2E9)
인간 BRAF 단백질을 인식하는 Mouse IgG2a 단클론 항체로, Western blot 및 ELISA에 적합합니다. 액상 형태이며 PBS 버퍼에 보존제가 포함되지 않습니다. Affinity chromatography로 정제되어 연구용으로 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1–5 µg/mL
- Publications: References
ELISA
- Tested Dilution: 0.3 ng/mL
- Publications: References
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E9 |
| Immunogen | BRAF (NP_004324, 138–231 a.a.) partial recombinant protein with GST tag (MW of GST tag alone: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
FQNPTDVARS NPKSPQKPIV RVFLPNKQRT VVPARCGVTV RDSLKKALMM RGLIPECCAV YRIQDGEKKP IGWDTDISWL TGEELHVEVL ENVP
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer and plays a role in postsynaptic responses of hippocampal neurons.
This protein regulates the MAP kinase/ERKs signaling pathway, affecting cell division, differentiation, and secretion. Mutations in B-Raf are associated with various cancers, including:
- Non-Hodgkin lymphoma
- Colorectal cancer
- Malignant melanoma
- Thyroid carcinoma
- Non-small cell lung carcinoma
- Adenocarcinoma of lung
A pseudogene located on chromosome X has been identified for B-Raf.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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